| 质粒名称: | pLenti PGK Puro DEST (w529-2) |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 19068 |
| 存储实验室: | Eric Campeau, Paul Kaufman |
|
载体骨架基本信息 |
|
| 载体名称: | p156RRL-sinPPT-CMV-GFP-PRE/Nhe I |
| 载体出品公司: | -- |
| 载体抗性: | 氯霉素和氨苄西林 |
| 空载体大小: | 9599 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，慢病毒 |
| 筛选标记: | 嘌呤霉素 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | PGKforw |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pLenti PGK Puro DEST (w529-2) was a gift from Eric Campeau & Paul Kaufman (Addgene plasmid # 19068 ; http://n2t.net/addgene:19068 ; RRID:Addgene_19068) |
| 参考文献部分: | A versatile viral system for expression and depletion of proteins in mammalian cells. Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, Campisi J, Yaswen P, Cooper PK, Kaufman PD.. PLoS One. 2009 Aug 6;4(8):e6529. 10.1371/journal.pone.0006529 PubMed 19657394 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
质粒图谱.png)
载体简介
载体序列
LOCUS pLenti PGK Puro DEST (w529-2) 9605 bp ds-DNA circular SYN 07-AUG-2019
DEFINITION 3rd gen lentiviral Gateway destination vector, expression, PGK
promoter, Puro.
ACCESSION .
VERSION .
KEYWORDS pLenti PGK Puro DEST (w529-2)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9605)
AUTHORS Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO,
Campisi J, Yaswen P, Cooper PK, Kaufman PD.
TITLE A versatile viral system for expression and depletion of proteins in
mammalian cells.
JOURNAL PLoS One. 2009 Aug 6;4(8):e6529.
PUBMED 19657394
REFERENCE 2 (bases 1 to 9605)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 7, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9605
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 26..130
/gene="bla"
/label=AmpR promoter
CDS 131..991
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(349..368)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 1162..1750
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 1651..1670
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 1904..1921
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 2038..2059
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 2074..2104
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 2112..2128
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 2117..2139
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 2136..2152
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 2136..2152
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 2171..2191
/label=T3
/note="T3 promoter, forward primer"
promoter 2173..2191
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
promoter 2219..2445
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
LTR 2446..2626
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 2673..2798
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 3291..3524
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 4020..4137
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 4183..4693
/label=hPGK promoter
/note="human phosphoglycerate kinase 1 promoter"
primer_bind 4612..4630
/label=hPGK-F
/note="Human PGK promoter, forward primer"
protein_bind 4748..4872
/gene="mutant version of attR"
/label=attR1
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
promoter 4897..4927
/label=lac UV5 promoter
/note="E. coli lac promoter with an ""up"" mutation"
CDS 4981..5640
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
primer_bind complement(5047..5066)
/label=CAT-R
/note="Chloramphenicol resistance gene, reverse primer"
CDS 5982..6287
/codon_start=1
/gene="ccdB"
/product="CcdB, a bacterial toxin that poisons DNA gyrase"
/label=ccdB
/note="Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains."
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
primer_bind 6166..6185
/label=ccdB-fwd
/note="ccdB gene, forward primer"
protein_bind complement(6328..6452)
/gene="mutant version of attR"
/label=attR2
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
misc_feature 6483..7071
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(6536..6556)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(6954..6965)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
promoter 7085..7584
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
primer_bind complement(7120..7143)
/label=MSCV-rev
/note="Murine stem cell virus, reverse primer"
primer_bind 7517..7536
/label=mPGK-F
/note="Mouse PGK promoter, forward primer"
CDS 7605..8204
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(7605..7624)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 8101..8121
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
LTR 8342..8575
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 8647..8768
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(8684..8703)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 8738..8757
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin 8808..8943
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 8870..8889
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(8963..8982)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(8964..8982)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(8992..9009)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(8992..9008)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(9001..9023)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 9150..9605
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(9237..9256)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 9447..9468
/label=F1ori-F
/note="F1 origin, forward primer"
ORIGIN
1 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
61 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
121 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
181 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
241 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
301 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
361 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
421 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
481 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
541 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
601 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
661 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
721 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
781 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
841 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
901 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
961 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
1021 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
1081 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
1141 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
1201 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
1261 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
1321 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
1381 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
1441 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
1501 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
1561 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
1621 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
1681 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
1741 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
1801 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
1861 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
1921 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
1981 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
2041 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
2101 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
2161 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg
2221 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
2281 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
2341 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
2401 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
2461 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
2521 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
2581 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
2641 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
2701 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
2761 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
2821 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
2881 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
2941 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
3001 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
3061 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
3121 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
3181 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
3241 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
3301 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
3361 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
3421 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
3481 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
3541 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
3601 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
3661 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
3721 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
3781 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
3841 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
3901 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
3961 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact
4021 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
4081 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttatc
4141 gatcacgaga ctagcctcga gaagcttgat atcgaattcc acggggttgg ggttgcgcct
4201 tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa
4261 acgcagcggc gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg
4321 gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg
4381 ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac
4441 tagtaccctc gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc
4501 tgtggccaat agcggctgct cagcagggcg cgccgagagc agcggccggg aaggggcggt
4561 gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc
4621 attctgcaag cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga
4681 cctctctccc cagggggatc caccggttcg tcgactagtc cagtgtggtg gaattctgca
4741 gatatcaaca agtttgtaca aaaaagctga acgagaaacg taaaatgata taaatatcaa
4801 tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa cacaacatat
4861 ccagtcacta tggcggccgc attaggcacc ccaggcttta cactttatgc ttccggctcg
4921 tataatgtgt ggattttgag ttaggatccg tcgagatttt caggagctaa ggaagctaaa
4981 atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa
5041 cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat
5101 attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt
5161 cacattcttg cccgcctgat gaatgctcat ccggaattcc gtatggcaat gaaagacggt
5221 gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa
5281 acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat
5341 tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag
5401 aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg
5461 gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc
5521 gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga tggcttccat
5581 gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcgtaa
5641 agatctggat ccggcttact aaaagccaga taacagtatg cgtatttgcg cgctgatttt
5701 tgcggtataa gaatatatac tgatatgtat acccgaagta tgtcaaaaag aggtatgcta
5761 tgaagcagcg tattacagtg acagttgaca gcgacagcta tcagttgctc aaggcatata
5821 tgatgtcaat atctccggtc tggtaagcac aaccatgcag aatgaagccc gtcgtctgcg
5881 tgccgaacgc tggaaagcgg aaaatcagga agggatggct gaggtcgccc ggtttattga
5941 aatgaacggc tcttttgctg acgagaacag gggctggtga aatgcagttt aaggtttaca
6001 cctataaaag agagagccgt tatcgtctgt ttgtggatgt acagagtgat attattgaca
6061 cgcccgggcg acggatggtg atccccctgg ccagtgcacg tctgctgtca gataaagtct
6121 cccgtgaact ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg
6181 atatggccag tgtgccggtc tccgttatcg gggaagaagt ggctgatctc agccaccgcg
6241 aaaatgacat caaaaacgcc attaacctga tgttctgggg aatataaatg tcaggctccc
6301 ttatacacag ccagtctgca ggtcgaccat agtgactgga tatgttgtgt tttacagtat
6361 tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat atcattttac
6421 gtttctcgtt cagctttctt gtacaaagtg gttgatatcc agcacagtgg cggccgctcg
6481 acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
6541 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
6601 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
6661 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
6721 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
6781 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
6841 tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc
6901 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
6961 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
7021 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaattc
7081 taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg
7141 ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt
7201 aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta
7261 gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta
7321 gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc
7381 ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga
7441 aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg
7501 tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc
7561 tcttcctcat ctccgggcct ttcgacctgc agcccaagct taccatgacc gagtacaagc
7621 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg
7681 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg
7741 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
7801 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
7861 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
7921 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg
7981 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
8041 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc
8101 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg
8161 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgacgcccg ccccacgacc
8221 cgcagcgccc gaccgaaagg agcgcacgac cccatgcatc tcgagggccc ggtaccttta
8281 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
8341 ctggaagggc tagctcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
8401 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
8461 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
8521 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
8581 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
8641 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
8701 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
8761 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa
8821 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
8881 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
8941 gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt
9001 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
9061 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
9121 acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
9181 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
9241 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
9301 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
9361 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
9421 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
9481 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
9541 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
9601 aattt
//
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